Single-cell electroporation (SCE) is a specialized technique allowing the delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. The distinct advantage of this technique is that experimental manipulations may be performed on individual cells while leaving the surrounding tissue unaltered, thereby distinguishing cell-autonomous effects from those resulting from global treatments. When combined with advanced in vivo imaging techniques...
so in part 2 we will discuss more, liek, How to use this on windows???! And how to use the glibc function!!! Also we will discuss some other techniquez!
Hello world! I thought it was about time that wonderhowto had its own World of Warcraft community. A place where gamers from all over the real world can come together to discuss the in and outs of the biggest and best MMORPG.
If you want more people to follow you on Google+, you'll have to work for it. Not everyone can be +Tom Anderson or +Robert Scoble overnight, you know.
Mike was lucky enough to have Leigh Whannell and James Wan drop by the Film Lab to discuss their new movie INSIDIOUS, which we loved back during Midnight Madness and opens Friday April 1st in theatres everywhere.
Visit www.studiorealism.com to see other tutorials on how to sculpt the human figure in clay. This is a deleted scene from my new beginning figure sculpting DVD about sculpting clay figures, which can be found at www.studiorealism.com
Visit www.studiorealism.com to see other tutorials on how to sculpt the human figure in clay. This is a deleted scene from my new beginning figure sculpting DVD about sculpting clay figures, which can be found at www.studiorealism.com
A translation via msnbc.comMeg Whitman says she's a different kind of Republican...But Pete Wilson is in charge of her campaign.Whitman attacks undocumented workers to win votes, but an undocumented woman worked in her home for nine years.She says she'll create jobs---but wants to eliminate forty-thousand state job, including teachers and nurses.Whitman says one thing in Spanish---and something different in English.The real Meg Whitman has no shame. She's a two-faced woman. Resource:http://ww...
Here is the official trailer for "The Social Network" which is David Fincher's film about FaceBook. The official website for this movie is http://500MillionFriends.com
Nutritionist, Chrissy Thums, discuss proper nutritional content snack choices on and off the field, in between games and post game.
Dr David Marshall, Director of Sports Medicine at Children's Healthcare of and Casey discuss the warning signs, prevention, and treatment of this growth plate injury.
Dr. David Marshall, Medical Director of Sports Medicine at Children's Healthcare of Atlanta discuss the warning signs, prevention, and treatment of Osgood Schlatter Disease.
Increasingly, I'm turning to Google+ as a source of news, and it looks like I'm not the only one. On Thursday, news of a small earthquake broke on both Twitter and Google+. One curious user, +Keith Barrett, decided to try and find out which social network was faster with the news. Turns out it was a tie. As Google integrates Google+ more closely with the rest of its services, and more users post relevant stories, I think we'll start to see Google+ as a place that can create and break stories,...
I’m seeing a flood of posts on youth football forums and even getting a few e-mails from youth football coaches complaining about “player agents” also known as parents. Most of these unhappy coaches are dealing with parents that have non-issues, have an agenda, are misinformed, don’t know much about the game of youth football or are just people that gain great pleasure from complaining. The 80/20 rule is more like 95/5 in youth football, 95 percent of the problems are caused by 5 percent of t...
Since Angry Birds is apparently an "indie game", here's an interesting tidbit. Changsa, China's Window of the World theme park recently added an especially zeitgeisty activity to their collection of diverse attractions: a real-life Angry Birds game, which allows participants to catapult Angry Bird "balls" at targets using an actual slingshot.
Learn Tagalog Today Episode 5, Body Parts ! In this lesson, we will be looking at how to say certain body parts in Tagalog. You might be visiting The Philippines when you experience some pain and need to communicate this with a doctor or nurse.
Learn Tagalog Today Episode 3,Enclitic Words!!! In today's lesson we look at some words called Enclitic Words and Enclitic Particles.
Learn Tagalog Today Episode 2,Domestic Dispute! In this lesson,follow along as we jump into a Domestic Dispute between boyfriend and girlfriend.
Description: Admit it, having thousands of valid email Ids give you the power to do anything! Such as adding thousands of friends to your Facebook, MySpace, Twitter, etc profiles or promoting your article or blog in just a single click and much more! But how do we do it? I searched all over the internet in a hope to get a perfect email producing software but ended with just two -
Welcome to our new world... Very Simply Put... this is a place safer than FACEBOOK! I know my personal friendships get tired of me posting so much about Crochet... It's my passion, my life, and my dream! SOOOO this place gives us a place to discuss, post, talk, and no one on Facebook has to be bothered by it! So join me if you want to talk Crochet... post your pictures... This is a far safer place! Have Fun! Michael
Welcome to the Ninja Saga world! Here you can talk about all things related to Ninja Saga. From tutorials to cheats, all types of posts and comments are welcome here (so as long as they are appropriate!)
In this two part lesson we'll discuss the differences between open chords and barre chords, then introduce the 4 main primary barre chords. I'll give a few tips on how you might position your hand to play the barre chords cleanly, and explain how each barre chord pattern relates to the guitar neck to produce a chord in all 12 keys. With these 4 patterns and some practice you should be able to play 48 chords. I'll suggest a couple of practice routines and give you a quick demo using "Light My ...
Tommy and Al dive into discussing bellflowers with special guests Eli and Brian. Tommy, woodworking master, demonstrates how he made the lines on the table leg. He then reviews how to make the bellflowers and Eli cuts them. Afterwards, Tommy and Brian discuss their furniture work so far.
STANDP'S WINDOWS 7 "ASTON-2" CUSTOMIZED DESKTOP IT ROCKS COMPLETELY!. DEC
The purpose of this article is to discuss how you can change your Windows 7 logon screen. This post will explain how to require a user to enter a username and password by default like the classic version of Windows.
Calling all curious minds—scientists, anthropologists, relentless tourists: Saturday, April 9th, is International Obscura Day, the day to "explore hidden treasures in your hometown," or so says Atlas Obscura, a website dedicated to public curiosities and esoterica. If you're the kind of person who appreciates public oddities every day of the year, tomorrow is icing on the cake. Celebrate Obscura Day in one of hundreds of locales—from Los Angeles to Sydney, from Berlin to Manila.
One of the objectives of the writings and other media items chosen or created for the Super Small Business World is to help small business managers and potential managers obtain access to information to begin the research needed to make key decisions. Another objective is to facilitative thinking in the lines of exploring possible opportunities. A third objective is to encourage the readers and learning participates that there is hope of a brighter future and that positive reality is within r...
Thanksgiving is less than a week away! We know - Thanksgiving can be a mess - too many relatives, too many dishes to cook, and too many things to do. At least no one can complain there's too much food to eat. This week, we'll show you how you can make your life easier with some innovative ideas.
For those of you familiar with our "The Very Important Dudes & Dudettes in Film History Show" video series (hosted by Matthew Price of the MAMO podcast) you'll be pleased to hear that MAMO & thesubstream.com have teamed up to bring you a more in-depth discussion about our favourite filmmakers every month, straight to your ear-holes!
It seems the Republicans aren't happy about Obama. But then again, they never will be. As the time for re-election draws near, the Republican Party is scrambling to chose their candidate who will beat Obama. So far, Mitt Romney (we'll discuss the candidates soon) has somewhat dominated the scene, with spurts of awesomeness from Rick Santorum and Newt Gingrich. Ron Paul stays steady, currently holding the second most number of delegates (yes, we'll go over terms also).
The recent report of the ubiquity of extrasolar planets coupled with the fact that it bodes well for searches for life friendly Earth twins, brought me back to my reading of Carl Sagan's 1966 Intelligent Life In The Universe and later article in Sky & Telescope where he came up with a remarkable number in favor of such.
In this Null Byte, I'm going to teach you about Null Byte Injections. Null Bytes are an older exploit. It works by injecting a "Null Character" into a URL to alter string termination and get information or undesirable output (which is desirable for the malicious user).
Google+ Hangouts are proving to be a proficient platform for teaching and communicating in real time with others. If you're someone who creates instructional content online, you may want to consider using the platform for your next project.
In case you haven't noticed, I absolutely adore video games. Most of my friends don't, so to get my fix of knowledgable video game conversation I have turned to podcasts. They're free, they feature the smartest people in games journalism, and can be enjoyed while doing just about anything. Working. Walking the dog. Crying yourself to sleep. Whatever you're into.
In the news this past couple of days, we've heard of about 10 Argentine hooligans being deported back to Argentina before the start of the World Cup. I'm glad for the South African authorities showing that they have a firm grasp of security leading up to Friday's kick-off, and that the inter-agency cooperation between countries was a serious one.
Optional sections to add to your resume can help fill in the gap where needed and enhance the skills and proficiencies already listed on your resume. Completion of this how to video will discuss the optional resume sections such as profile, technical skills, certifications, awards, memberships and community involvement. Make your own amazing resume today.
Last time, we looked at archaic cryptography, so you should have a basic understanding of some of the concepts and terminology you'll need. Now, we'll discuss one of the most important advances in computer security in the 20th century—public key cryptography.
Cryptography is a quintessential part of computer security in the modern world. Whenever you buy something on eBay or log into Facebook, that data is encrypted before it's sent to the server in order to prevent third parties from eavesdropping and stealing your sensitive information.
We're officially seeking Null Byters interested in teaching others! Contributors will write tutorials, which will be featured on the Null Byte blog, as well as the front page of WonderHowTo (if up to par, of course). This is a job meant for anyone with the will to share knowledge. There is no need to be intimidated if you fear you lack the writing skills. I will edit your drafts if necessary and get them looking top-notch! You can write tutorials of any skill level, and anything you feel like...
A diary or personal blog is a great way to recapture the days behind you, but to a photographer, the best way to remember the glory days is with a picture—one single photograph that best describes the day and your mood at the time. Do it everyday for a year and you'll have 365 pictures that will not only bring a smile to your face, but will help you understand what was important to you in life.
