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How To: Perform single cell electroporation of neurons

Single-cell electroporation (SCE) is a specialized technique allowing the delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. The distinct advantage of this technique is that experimental manipulations may be performed on individual cells while leaving the surrounding tissue unaltered, thereby distinguishing cell-autonomous effects from those resulting from global treatments. When combined with advanced in vivo imaging techniques...

How To: Do a single leg deadlift with a medicine ball

If you want to improve balance and strength in the leg muscles, try the single leg deadlift (also called the single leg Romanian deadlift). This exercise is primarily going to work your hamstrings and butt. The single leg deadlift comes with a high degree of difficulty because it is performed on one leg. An advanced level of balance and muscular control is required for this exercise. Here are the instructions:

How to Be Your Own SpaceX: Design, Build & Test Liquid-Fueled Rocket Engines

Move over NASA— SpaceX is taking over. Well, not really. But today, the privately funded spacecraft company broke all expectations when their Dragon capsule fell to a soft landing in the Pacific Ocean, completing an undoubtedly successful demo flight of nearly two full trips around Earth. It was the first re-entry of a commercial spacecraft ever, bringing commercial space transportation closer to reality.

News: The Federal Government Is Spying on Every Single American, Say NSA Whistleblowers

Two former high-ranking officials at the U.S. National Security Agency (NSA), a federal bureaucracy that collects data and intelligence on foreign communications for national security purposes, have come forward with allegations that the NSA actively monitors Americans as well. According to testimonies from both Thomas Drake, a former NSA senior official, and Kirk Wiebe, a former NSA senior analyst, the agency actively monitors and collects intelligence on every single American as part of a m...

How To: Calculate the Cost of Driving

With gas prices soaring in the US and around the globe, the cost to drive to a vacation destination is becoming a much larger expense item in the overall travel budget. Although it's easy to see that gas prices have risen dramatically in the past several months, it's not nearly as easy to figured out how it will impact that upcoming car trip, so here are the items you need to take into consideration to calculate how much it will cost to drive to a destination.

How To: Make a perfectly poached egg for breakfast

The key to a perfectly poached egg? A soft touch and a little bit of vinegar. This video shows you the technique for poaching an egg so you can get it right every single time. Now all you need is some Hollandaise sauce, and you're ready to make Eggs Benedict! Make a perfectly poached egg for breakfast.

How To: Make a light and refreshing cucumber rum cocktail

Another great summer drink! The sweetness of fresh, ripe cucumber goes well with the rum. For this drink, you will need: 1 slice of cucumber, small cucumber cubes, 2 ounces single barrel rum, 1/2 ounce lemon juice or passion fruit juice, 1/2 ounce honey syrup, ice and another slice of cucumber for garnish. Make a light and refreshing cucumber rum cocktail.

How To: Disable and uninstall extensions in the Safari web browser

If you're wanting to turn off an extension in Safari, this short video will show you what needs to be done. You'll see how to disable and uninstall single extensions, as well as how to turn off all extensions, in the Safari web browser. It's a simple process— you just need to know where to go— preferences. Disable and uninstall extensions in the Safari web browser.

How To: Use group masks in modo 301

This 3D software tutorial teaches you the power and simplicity of the group mask in modo 301. When you hear the term mask, you probably think of layer masks, but group masks are different and more complex. See how to use a single procedural, image or vertex map texture to modulate multiple complex material and texture sets in modo 301. Use group masks in modo 301.

News: Chin Chin - We Don't Wanna Be Prisoners 7" single

Fantastic reissue of this all female punk group from Switzerland. Formed in the early 80's, this was their first single released on their own label Farmer Records. Now Portland's Mississippi Records has finally released this obscure classic. Their sound is a cross between punk and twee pop. Catchy melodies with sing along choruses, upbeat tempos, thumping bass lines and noisy post punk guitars. Very much in the same vein as bands like the Shop Assistants (whom they toured with), Kleenex/Lilip...

How To: Double Uni Knot

This shows how to tie a Double Uni Knot. The Double Uni Knot is used to join lines of similar of different diameters. It is based on the same system as a normal "single" uni knot. So if you learn the system you will be able to join lines or attach the line to end tackle with the same "knot system". Double Uni Knot.

How To: Get a horse to take dewormer medication

Watch as Janice Posnikoff, DVM, shows how to get your horse to take his dewormer medication. Control harmful, performance robbing parasites including encysted small strongyles and tapeworms in a single dose with QUEST (moxidectin) and QUEST PLUS (moxidectin/praziquantel). Learn how to give a horse deworming medication by watching this video tutorial and following along with equine veterinarian Janice Poskinoff. Get a horse to take dewormer medication.

How To: Exercise your triceps using weights

Chris Cooke explains three exercises you can do to exercise your triceps using weights. You can do triceps dips, lying triceps extension, and single overhead press. It is recommended that you do ten repetitions of each exercise. These exercises require two dumbells and a bench. Exercise your triceps using weights.

How To: Exercise your calves with free weights

Chris Cooke demonstrates how you can exercise your calves with free weights. You can do the following: standing calf raise, single calf raise, and seated calf raise. It is recommended that you do 15 repetitions of each exercise. These exercises require dumbbells,

How To: Get a perky butt

Sophie Rycott demonstrates how you can get a perky butt by doing pilates. There are four exercises you can do: hamstring press, side leg kicks, leg extensions, and single leg kicks. Get a perky butt.

How To: Dock your boat

Graphical animations show you to dock twin screw and single screw vessels. Additional tips for all skippers who face the stomach churning tasks of bringing their pride and joy into dock. Dock your boat.

Making Electromagnetic Weapons: The Theory Behind EMP Generators

This is the third part of my electromagnetic pulse series (see Part One and Part Two). By now, I've covered the hardware and general concept of electromagnetic pulse generators, but how exactly do they disable electronics? How can an invisible field of energy have such a catastrophic effect on computers, cell phones, and most any other electronics? I'll be answering all these questions in part three of Making Electromagnetic Weapons.

How To: Do a single leg bridge on a swiss ball

If traditional supine bridges become too easy, you can try to progress to the one leg version. This exercise is going to work your glutes like none other. Your hamstrings and lower back are also heavily engaged during this exercise. The single leg bridge requires an advanced level of fitness. This is generally true in cases where one leg is being used to support the majority of your bodyweight. Another factor that makes this exercise difficult is that it is perform using a Swiss ball (unstabl...