Single-cell electroporation (SCE) is a specialized technique allowing the delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. The distinct advantage of this technique is that experimental manipulations may be performed on individual cells while leaving the surrounding tissue unaltered, thereby distinguishing cell-autonomous effects from those resulting from global treatments. When combined with advanced in vivo imaging techniques...
Welcome to Minecraft World! Check out our advanced tutorials and come play on our free server. Missed the other guides? Click on the links!
Do you love video games? Would you devote your free time to creating your own game—one superior to the games you already have? Or at least one that has more Neil Patrick Harris jokes?
Christmas is just a few days away, which means everyone is out shopping for last minute gifts. But wait— you still need some Christmas cards! The stores are going to be packed and Hallmark's going to have huge lines— so now what?
Most of your who visit Fear of Lightning are probably well familiar with laser weaponry, thanks to Christopher's three-part series covering carbon dioxide, flashlamp, and semiconductor lasers. Another type of laser currently being developed as a weapon is the fiber laser, which is compact and efficient, but much weaker than a chemical laser. Fiber lasers are more commonly used in laser cutting and marking, telecommunications, spectroscopy, and of course... music.
What comes to your mind when you see the words 'crowd control'? A mob of rioters shouting, throwing rocks & molotovs, wrecking cars, raiding homes, holding signs.......Sound familiar?
In this article, I'll show you how you can make your very own bullet/shell bottle opener. All you need is a .50 caliber deactivated round and some workshop tools!
Ribbing on double pointed needles is quite simple. Here's what to do:
It'd be nice to see what a penny will buy these days. Maybe you could draw something showcasing the many uses still available today for a penny (if any). With gas prices rising, it'd be nice to know you can still get something out of one cent.
I'm sure at this point you're all crafting away merrily, frantically searching for bushels, and trying to keep track of what you need, so I made a chart for each building and their recipes that is simple and easy to reference.
Sometimes ghetto is the way to go. Yankee ingenuity is always the way to go (at least on WonderHowTo, that is).
Designer Mike Clare (of my own alma mater, RISD) has extended the augmented reality mania (1, 2, 3) to baked goods. Thanks to the crowd and Josh Delcore at AR World for the find. Here's how it works, via Design Boom:
Today's WSJ takes a tour of Russian billionaire Andrey Melnichenko's 394-foot insanely luxe yacht, coined "A".
Chase the winter blues away by adding bright splashes of color to your boring winter tights. Using inexpensive nylon tights, fabric paints and basic craft supplies, you can do pretty much anything to transform your neutral-colored tights into something more colorful and fun.
Selfies have been around for quite a while, but with the popularity of social media (in particular Instagram) they've become ubiquitous in our culture. There's even mobile apps dedicated solely to selfies. If that and your news feed isn't proof enough, just check with the Oxford Dictionary.
Originally made using whale fat, candles first appeared over 2,200 years ago as a means of illumination. From the 1st century up until the 19th century, candles were primarily made using beeswax or tallow, and aside from providing light, were used as a method of keeping time.
If the annual tradition of buying a new wall calendar at a heavily discounted price at some mall kiosk by the end of January is not exactly your scene, then how about making your own nifty DIY wall calendar for 2013?
Need to look like a rotting, decaying zombie corpse for Halloween? Rather than bribing your special effects makeup artist friend to treat you one for the night, you can definitely DIY a couple of good Halloween makeup effects using common household items in your pantry or medicine cabinet.
Itching to make your own guerrilla-style street art on the side of buildings, freeway overpasses, and abandoned billboards? The beauty of street art is that you don't need an expensive canvas or frame to display your creative expression.
Knitted balls can be used for all sorts of projects. The obvious is a Christmas ornament, but knitted balls can also be the basis for knitting toys or snowmen. And of course, they can be used simply as balls which make a lovely gift for a baby. They can be all one color, striped or patterned. To get started, follow the directions below for a one-color knitted ball. You will need to know how to knit on double-pointed needles, so if you need help with that, check out my other article.
You'll be so glad to know how to KNIT THIS LITTLE PURSE. You'll want to make one for everyone you know. You can start and finish it in a little over an hour. You can knit it all one color and embellish it with buttons (see photo below) or you can make stripes (as on the video below) or use two colors (as on the photo above). You can make it in a flat stockinette stitch or something more interesting like the Raspberry Stitch. Do what you want and just have fun.
For an object to balance, you have to have the center of gravity directly above the base. Well, reverse that to see that you can also balance an object by having the center of gravity directly below its base.
Animals and insects make great cartoon characters. So get ready to learn how to draw a worm that turns into a bookworm. All you need is paper, pencil, marker, colored chalks or colored pencils, and an eraser. Start by drawing a squiggly line. Come around and double the line, keeping an equal distance from the other. Draw a curved shape for a head at the end. Draw large circles for his eyes and connect them to give him glasses. Draw his pupils going all the way to the right. Add two small eyeb...
Did you ever think a question mark could turn into a cartoon? Well, grab your pen because Bruce Blitz is going to show you how you can create cartoons from symbols. This is an easy way to learn to draw cartoons. All you need is paper, pencil, markers, colored chalks or colored pencils, and an eraser. First, draw a question mark. Next, draw 2 C’s (one backwards) for eyes at the top point. The curve in the question mark will become a nose. Add 2 pupils and eyebrows facing up. Draw hair and an e...
Fans of Doctor Who know that the sonic screwdriver is the ultimate tool. It can unlock doors, turn on lights, detect life forms, solder wires, and sabotage weapons.
They can be called wash rags, wash cloths or dish cloths. There are lots of different patterns for them. Here's an easy washcloth that incorporates the Stockinette Stitch with Seed Stitch edging.
Do you have a friend who's a vegetarian? Well, this prank is perfect for them. It's pretty simple, where the objective is to tamper with their favorite bottle of salad dressing, and no... it's not the loosened cap prank, but it does have a similar result. You Will Need:
Mobile devices can do just about anything these days, thanks to third-party developers. iPhones and Android devices have been known to do some pretty wild things. Need a dupe key made? Scan and order one with your iPhone. Want to know if you're hotter than Justin Bieber? Compare your facial features. Are you a policeman who needs to ID a suspect? Scan their fingerprints and irides. Want to control your Canon DSLR remotely? Use your Android phone.
Here are just a few little random tips that may help you while making balloon animals for fun and profit. This list is only a collection of random balloon tips and tricks that I've come up with on the fly tonight. If you have any other suggestions or questions, please feel free to post them in the comments section and I'll do what I can to help as well.
Technology: The Less You Know the Better?
Another media player bites the dust. First, we saw the extinction of the phonograph and gramophone. Then came the fall of eight-track tapes. Even modern record players became more and more obsolete, with DJs switching over to the world of digital music. And today— the end of cassette players! What's next? Compact discs?
Take one drunk person passed out with shoes on Add a couch
In this article, you'll learn how to create deconstructed roses in less than five minutes with your leftover fabric scraps. It's a great way to recycle your leftover fabric! Follow the instructions below and watch the videos for a demonstration.
Welcome to Minecraft World! Check out our advanced tutorials and come play on our free server. Part 5 in Minecraft World's Ultimate Survival Guide is, for me, the longest article to write. Since I squeezed out a lot of information from other areas to put into survivability, its going to be a fairly long read. Of course, you can just skip to your topic of choice.
There's a time in everyone's life when they need to become immortalized and reprographed in a full-blown comic spread, complete with halftone dots and speech bubbles—or squares, if you're a square. With our favorite program Photoshop, you can quickly turn any hyperbolic celebrity into a comical farce. Even normal people can become vintage villains with a little pixelation.
Each year on Shrove Tuesday and Ash Wednesday, Ashbourne becomes a war zone! The majority of the ablebodied men, women and children take to the streets to play what is probally the largets football game in the world! - The two teams number in the hundreds, and the palying field is 3 miles long, 2 miles wide and has the town of Ashbourne in the middle!
I'm starting a series on the top astronomers, with probably about eleven astronomers that I will be covering overall. So, let's start out from the top, with the top most important astronomer. In my opinion, Galileo Galilei is the top astronomer.
Continued from Organize Yo'sef Part One!
When it comes to social engineering, Null Byte is here to show you how it's done. Social engineering is the key fundamental to unlocking tons of possibilities and opportunities in your everyday life. So, what is social engineering? Social engineering can be called many things. It's taking nothing and turning it into something. It's taking the bad and making it good. Above everything else, it's the art of manipulating the world and people around you—coercing that salesman into giving you a low...
As Google+ became available to more people, it started running into issues of accounts being suspended due to names that did not sound "real". According to Google+'s community standards, names that users "commonly go by" were encouraged, but not pseudonyms. Those who were using pseudonyms in their Google+ profiles quickly found themselves suspended from using Google+.
