Single-cell electroporation (SCE) is a specialized technique allowing the delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. The distinct advantage of this technique is that experimental manipulations may be performed on individual cells while leaving the surrounding tissue unaltered, thereby distinguishing cell-autonomous effects from those resulting from global treatments. When combined with advanced in vivo imaging techniques...
German photographer Peter Langenhahn's process is incredibly laborious; similar to photographer Lori Nix, he can spend up to three months on a single photograph. But while Nix spends the time constructing elaborate sets, Langenhahn is anchored to the computer, editing.
This single malt whisky is made from type two diabetics' urine, who excrete more sugar than healthier people. The Gilpins are trying to make a statement about the environment, resources, and other worthy causes, but I can't get past the urine.
Search "Pilates animation" on Wonderhowto for other more videos by this user. If you are looking a for a quick pilates reference, take a look at this pilates animation. This a quick & easy, clearly explained diagram of the pilates mat exercise: Classical Pilates for Beginners:
If you want to improve balance and strength in the leg muscles, try the single leg deadlift (also called the single leg Romanian deadlift). This exercise is primarily going to work your hamstrings and butt. The single leg deadlift comes with a high degree of difficulty because it is performed on one leg. An advanced level of balance and muscular control is required for this exercise. Here are the instructions:
Staking keeps your garden beautiful by keeping mature flowers from flopping under their own weight. Garden designer and author Tracy DiSabato-Aust will show you how to stake tall, slender flowers. Stake single stemmed flowers.
Two former high-ranking officials at the U.S. National Security Agency (NSA), a federal bureaucracy that collects data and intelligence on foreign communications for national security purposes, have come forward with allegations that the NSA actively monitors Americans as well. According to testimonies from both Thomas Drake, a former NSA senior official, and Kirk Wiebe, a former NSA senior analyst, the agency actively monitors and collects intelligence on every single American as part of a m...
See how to use Dreamscape to generate foam, how to export it as a bitmap sequence and how to control every single parameter to change its look. See how to use 3ds Max 2010's built-in grouping features to work on multiple meshes the same way you might work on a single object. Whether you're new to Autodesk's popular modeling software or a seasoned 3D artist just looking to better acquaint yourself with the application, you're sure to be well served by this video tutorial. For more information,...
The key to a perfectly poached egg? A soft touch and a little bit of vinegar. This video shows you the technique for poaching an egg so you can get it right every single time. Now all you need is some Hollandaise sauce, and you're ready to make Eggs Benedict! Make a perfectly poached egg for breakfast.
Another great summer drink! The sweetness of fresh, ripe cucumber goes well with the rum. For this drink, you will need: 1 slice of cucumber, small cucumber cubes, 2 ounces single barrel rum, 1/2 ounce lemon juice or passion fruit juice, 1/2 ounce honey syrup, ice and another slice of cucumber for garnish. Make a light and refreshing cucumber rum cocktail.
If you're wanting to turn off an extension in Safari, this short video will show you what needs to be done. You'll see how to disable and uninstall single extensions, as well as how to turn off all extensions, in the Safari web browser. It's a simple process— you just need to know where to go— preferences. Disable and uninstall extensions in the Safari web browser.
This 3D software tutorial teaches you the power and simplicity of the group mask in modo 301. When you hear the term mask, you probably think of layer masks, but group masks are different and more complex. See how to use a single procedural, image or vertex map texture to modulate multiple complex material and texture sets in modo 301. Use group masks in modo 301.
This shows how to tie a Double Uni Knot. The Double Uni Knot is used to join lines of similar of different diameters. It is based on the same system as a normal "single" uni knot. So if you learn the system you will be able to join lines or attach the line to end tackle with the same "knot system". Double Uni Knot.
Searching for a Valentine's Day gift? Great. That means you have someone to share V-Day with this coming Monday. And you want to get the best gift to show your love. But c'mon—no matter what you get, it's probably enough to woo your lover over, even if it's as simple as a battery and magnet.
YouTuber sockcornnoodles is a punk. Not only does he have the best user name ever, but he's able to make it through a single chapter of Super Meat Boy without dying. Which also got him the 'Iron Boy!' achievement, worth a measly 10G on Xbox 360.
Fantastic reissue of this all female punk group from Switzerland. Formed in the early 80's, this was their first single released on their own label Farmer Records. Now Portland's Mississippi Records has finally released this obscure classic. Their sound is a cross between punk and twee pop. Catchy melodies with sing along choruses, upbeat tempos, thumping bass lines and noisy post punk guitars. Very much in the same vein as bands like the Shop Assistants (whom they toured with), Kleenex/Lilip...
In modo 301 3D painting has really "come of age". One powerful enhancement is the ability to paint across multiple images of varying resolution with a single stroke. It's very nice. Why are you reading this? Watch the modo 301 clip! Paint across multiple UVs in modo 301.
Watch as Janice Posnikoff, DVM, shows how to get your horse to take his dewormer medication. Control harmful, performance robbing parasites including encysted small strongyles and tapeworms in a single dose with QUEST (moxidectin) and QUEST PLUS (moxidectin/praziquantel). Learn how to give a horse deworming medication by watching this video tutorial and following along with equine veterinarian Janice Poskinoff. Get a horse to take dewormer medication.
This simple video crocheting tutorial shows how to do the half double crochet stitch. The half double crochet stitch is larger than the single stitch but still smaller than the double crochet stitch. Watch and learn how to crochet the half double stitch. Crochet a simple half double crochet stitch.
Changing the oil is the single most important thing you can do to prolong engine life. Why pay a garage to do it? It's easy when Haynes shows you how. Change your car's oil and filter.
Learn how to change the HTML ExpressShow template for single output, non-SCORM productions. The HTML template appears as the background around the video. ExpressShow is a video branding option within Camtasia Studio 5. Customize the HTML template for ExpressShow production.
We show you how to pitch a single pole Laser Competition tent. This is a must-see video for all those camping lovers as we show you exactly how to pitch a tent effectively. Pitch a terra nova laser competition tent.
Create and customize players using single title and multi-title template options. This tutorial also covers the basics of player distribution. Create players on Brightcove.
The team from BikeSkills show you the set up and technique you need to get the most out of your trail time. One of the important things is setting up your bars to allow for single finger braking. Dial in your ride.
This is one of Al Ward's favorite tricks - how to create a Glass Sphere using a single photo in Photoshop CS2. Learn to use Polar Coordinates to turn a photo into a sphere. You will also need tools like Dodge and Burn to adjust the overall effect. Create a glass sphere using a photo in Photoshop CS2.
Chris Cooke explains three exercises you can do to exercise your triceps using weights. You can do triceps dips, lying triceps extension, and single overhead press. It is recommended that you do ten repetitions of each exercise. These exercises require two dumbells and a bench. Exercise your triceps using weights.
Chris Cooke demonstrates how you can exercise your calves with free weights. You can do the following: standing calf raise, single calf raise, and seated calf raise. It is recommended that you do 15 repetitions of each exercise. These exercises require dumbbells,
Sophie Rycott demonstrates how you can get a perky butt by doing pilates. There are four exercises you can do: hamstring press, side leg kicks, leg extensions, and single leg kicks. Get a perky butt.
Tips for dealing with flaking lines. Good for both single handing and strong winds. Deal with flaking lines on your boat.
Graphical animations show you to dock twin screw and single screw vessels. Additional tips for all skippers who face the stomach churning tasks of bringing their pride and joy into dock. Dock your boat.
We have a soft spot for bar tricks...in the same way we love magic. These are two variations on a single theme. Get a dollar out from under an upside down beer bottle without touching nor toppling the bottle.
When replacing an old faucet there are generally three things to consider: how many holes your sink has, how far apart they are, and whether or not you will need to replace the drain.
If you haven't participated in this week's Math Craft project on the platonic solids, maybe this will inspire you to do so.
If traditional supine bridges become too easy, you can try to progress to the one leg version. This exercise is going to work your glutes like none other. Your hamstrings and lower back are also heavily engaged during this exercise. The single leg bridge requires an advanced level of fitness. This is generally true in cases where one leg is being used to support the majority of your bodyweight. Another factor that makes this exercise difficult is that it is perform using a Swiss ball (unstabl...
Cooks Illustrated has provided me some fantastic fundamentals in cooking. My grandma started buying me subscriptions to the magazine years ago and not a single publication has had the pages un-turned. Now I use their website as well to easily tag recipes and other helpful info to aid in my future cooking endeavors.
One day, California wakes up and every single Latino has inexplicably disappeared. No business owner, doctor, nurse, soldier, teacher, entertainer, athlete or politician can be found. No bus driver, farm worker, cook, gardener or nanny. All gone. California -- the ninth largest economy in the world -- grinds to a halt because Latinos have vanished. Chaos and tragedy follow.
